So, lets imagine that this is the gel and we add the dna fragments to different wells. Southern blotting animation please like, comment, share and subscribe category. So, a southern blot basically allows you to visualize a specific piece of dna that youre interested in. Northern blotting technique step by step this lecture explains about the northern blotting procedure and technique to identify specific rna fragment from a mixture of cellular rna molecules. So in this video, im gonna be talking about something known as a southern blot. This article also offers a southern blot protocol and a northern blot protocol. A free powerpoint ppt presentation displayed as a flash slide show on id. Southern blots with alkaline transfer koch institute for integrative.
If you have any questions about issues, please report us to resolve them. Jacqueline jai southern blot southern blot a piece nitrocellulose paper containing spots of dna ready for identification by a suitable molecular probe. Journey inside a cell as you follow proteins in cell signals. The method is named after its inventor, the british biologist edwin southern. Ppt southern blotting powerpoint presentation free to. A southern blot is a method used in molecular biology for detection of a specific dna sequence. So the fragments are gonna move down the gel and theyre gonna basically. Find highquality southern blotting stock photos and editorial news pictures from getty images. And theyre ready for you to use in your powerpoint presentations the moment you need them. Western blotting part i the first western blotting animation in a series of three animations will cover protein isolation. The animations are all available under a creative commons attributionnoncommercialsharealike 4. Southern, northern and western blotting southern blotting the random hexamer labeling process produces a radioactive singlestranded dna copy of both strands of the. Southern blotting this lecture explains about the southern blotting procedure and technique to identify specific dna fragment from a mixture of dna molecules separated by the agarose gel.
Tray with a stack consisting top down of a weight, paper towels, membrane of nitrocellulose or nylon. Southern blotting outline dna specimen collection and storage procedure watchpoints uses dna each individuals unique genetic blueprint is stored in material known as dna. Imran fakhroni nurkholis nugroho nino radiansyah indra ardi fauzi arfita sari southern blotting the technique was developed by e. Blotting methods free download as powerpoint presentation. The free label unincorporated probe can be removed by passing the probe mix through g25.
Blotting techniques the principle of western blotting show description show tags show categories for webmasters description. Dnalc animations feature stunning visualizations of cellular and molecular processes. An introduction to both northern and southern blotting, popular methods for the transfer of. Gel electrophoresis, techniques, dna isolation, agarose return to animation menu. Download premium images you cant get anywhere else. A full and complete explanation about western blotting technique. The southern blot is used to detect a specific dna sequence in dna samples. Western blotting is a powerful technique utilized by many researchers to identify the presence of specific proteins in. Southern blotting this lecture explains about the southern blotting. Worlds best southern blotting stock pictures, photos, and. Ppt southern, northern and western blotting powerpoint.